Anticorps Monoclonal anti-LDLR

• Phare
• Validé par KD/KO

LDLR Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A2A3

N° de cat : 66414-1-PBS

Synonymes

1A2A3, FH, FHC, LDL receptor

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Galerie de données de validation

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  WB analysis of HeLa using 66414-1-Ig (same clone as 66414-1-PBS)

  WB result of LDLR antibody (66414-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDLR transfected HeLa cells. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66414-1-Ig (same clone as 66414-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66414-1-Ig (same clone as 66414-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66414-1-Ig (same clone as 66414-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66414-1-Ig (same clone as 66414-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66414-1-Ig (same clone as 66414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66414-1-Ig (same clone as 66414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66414-1-PBS cible LDLR dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LDLR Protéine recombinante Ag1236
• Nom complet: low density lipoprotein receptor
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 100-160 kDa
• Numéro d’acquisition GenBank: BC014514
• Symbole du gène: LDLR
• Identification du gène (NCBI): 3949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

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