Anticorps Recombinant de lapin anti-LDLR

LDLR Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1O11

Avis (1)

N° de cat : 82724-1-RR

Synonymes

1O11, FH, FHC, LDL receptor

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Galerie de données de validation

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  WB analysis using 82724-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82724-1-RR (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 82724-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82724-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82724-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDLR antibody (82724-1-RR, Clone: 1O11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 82724-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody (82724-1-RR, Clone: 1O11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82724-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82724-1-RR against Human LDLR were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82724-1-RR cible LDLR dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LDLR Protéine recombinante Ag1236
• Nom complet: low density lipoprotein receptor
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 100-160 kDa
• Numéro d’acquisition GenBank: BC014514
• Symbole du gène: LDLR
• Identification du gène (NCBI): 3949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ioana (Verified Customer) (08-28-2025)

It worked very well for immunofluorescence, allowing colocalization with early endosomal markets.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HeLaM

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