Anticorps Recombinant de lapin anti-Leptin

Leptin Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240720A6

N° de cat : 84072-5-RR

Synonymes

LEP, 240720A6, OB, Obese protein, Obesity factor

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Galerie de données de validation

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  IF Staining of 3T3-L1 using 84072-5-RR

  Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using LEP antibody (84072-5-RR, Clone: 240720A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 84072-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LEP Recombinant antibody (84072-5-RR, Clone:240720A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HepG2 using 84072-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LEP Recombinant antibody (84072-5-RR, Clone:240720A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84072-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84072-5-RR against Human LEP were performed. The affinity constant is 35.3 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules 3T3-L1,
• Résultats positifs en FC (Intra): cellules HeLa, cellules HepG2

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84072-5-RR cible Leptin dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Leptin Protéine recombinante Eg0834
• Nom complet: leptin
• Masse moléculaire calculée: 167 aa, 19 kDa
• Numéro d’acquisition GenBank: BC060830
• Symbole du gène: Leptin
• Identification du gène (NCBI): 3952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Leptin is a product of the obese (ob) gene and, following synthesis and secretion from fat cells in white adipose tissue, binds to and activates its cognate receptor, the leptin receptor (LEP-R) (PMID: 34084149). Still, smaller quantities have been detected in other body tissues, including the brown adipose tissue (BAT), placenta, fetal tissue, stomach, muscles, bone marrow, teeth, and brain (PMID:7568067, PMID: 12086939). Leptin circulates in the blood in both free and protein-bound forms, where the free form of leptin is the biologically active form (PMID: 12086939). Leptin can enter the central nervous system (CNS) (in the area of the choroid plexus) by receptor-mediated transport (PMID: 29254602). Leptin secretion is higher in subcutaneous than in visceral adipose tissue (PMID: 11133069, PMID: 14726444).

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