Anticorps Polyclonal de lapin anti-P3H3

• Phare
• Validé par KD/KO

P3H3 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 16023-1-AP

Synonymes

GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B

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Galerie de données de validation

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  WB analysis using 16023-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16023-1-AP (P3H3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 16023-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 16023-1-AP (P3H3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-453s using 16023-1-AP

  IP Result of anti-P3H3 (IP:16023-1-AP, 4ug; Detection:16023-1-AP 1:500) with MDA-MB-453s cells lysate 2800ug.

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, cellules MDA-MB-453s, tissu ovarien de rat
• Résultats positifs en IP: cellules MDA-MB-453s

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

16023-1-AP cible P3H3 dans les applications de WB, IP et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P3H3 Protéine recombinante Ag8850
• Nom complet: leprecan-like 2
• Masse moléculaire calculée: 736 aa, 82 kDa
• Poids moléculaire observé: 82-90 kDa
• Numéro d’acquisition GenBank: BC017217
• Symbole du gène: LEPREL2
• Identification du gène (NCBI): 10536
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.

Publications

Species	Application	Title
mouse	WB,IP	PLoS Genet; Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.; Authors - Melissa E Heard
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
human	WB	Sci Rep; Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates.; Authors - Bernd Stratmann
MOUSE	WB	J Biol Chem; P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.; Authors - David M Hudson ; KO Validated
mouse	WB	J Biol Chem; Type I and type V procollagen triple helix use different subsets of the molecular ensemble for lysine post-translational modifications in the rER.; Authors - Yoshihiro Ishikawa
mouse	WB	AIMS Genet; Expression characterization and functional implication of the collagen-modifying Leprecan proteins in mouse gonadal tissue and mature sperm.; Authors - Sarah M Zimmerman

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (12-16-2021)

This antibody did not detect any band in RPE1 or MEF cell lysates

• Applications: Western Blot