Anticorps Monoclonal anti-LEPREL2

LEPREL2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F6F3

N° de cat : 68299-1-PBS

Synonymes

GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B

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Galerie de données de validation

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  WB analysis of HeLa using 68299-1-Ig (same clone as 68299-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 68299-1-Ig (same clone as 68299-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 68299-1-Ig (same clone as 68299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 68299-1-Ig (LEPREL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68299-1-PBS cible LEPREL2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LEPREL2 Protéine recombinante Ag8850
• Nom complet: leprecan-like 2
• Masse moléculaire calculée: 736 aa, 82 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC017217
• Symbole du gène: LEPREL2
• Identification du gène (NCBI): 10536
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.

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