Anticorps Polyclonal de lapin anti-LGALS3BP

• Phare
• Validé par KD/KO

LGALS3BP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 10281-1-AP

Synonymes

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

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Galerie de données de validation

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  WB analysis using 10281-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10281-1-AP

  WB result of LGALS3BP antibody (10281-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LGALS3BP transfected HepG2 cells.

• 

  WB analysis of fetal human brain using 10281-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10281-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10281-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10281-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10281-1-AP

  IP Result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:300) with HepG2 cells lysate 3480ug.

• 

  IP experiment of HEK-293 using 10281-1-AP

  IP Result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:400) with HEK-293 cells lysate 1000ug.

• 

  IHC staining of human oesophagus cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10281-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10281-1-AP (LGALS3BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules COLO 320, cellules HEK-293, cellules HepG2, tissu cérébral humain fœtal, tissu de lait humain, tissu plasmatique humain
• Résultats positifs en IP: cellules HepG2, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Informations sur le produit

10281-1-AP cible LGALS3BP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LGALS3BP Protéine recombinante Ag0294
• Nom complet: lectin, galactoside-binding, soluble, 3 binding protein
• Masse moléculaire calculée: 585 aa, 65 kDa
• Poids moléculaire observé: 65-90 kDa
• Numéro d’acquisition GenBank: BC002403
• Symbole du gène: LGALS3BP
• Identification du gène (NCBI): 3959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Publications

Species	Application	Title
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	IHC	Cancer Res; A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.; Authors - Silverman Ayaka M AM ; KD Validated
human	IF	Blood Adv; Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT.; Authors - Yu Akahoshi
mouse	WB	PLoS Pathog; Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.; Authors - Gang Xu
human	WB	Thromb Haemost; Proteomic discovery of 21 proteins expressed in human plasma-derived but not platelet-derived microparticles.; Authors - Smalley David M DM
human	IHC	Cancer Sci; MZT2A promotes NSCLC viability and invasion by increasing Akt phosphorylation via the MOZART2 domain.; Authors - Huanxi Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

Beautiful antibody - produces a single band at the correct size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: LNCaP

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