Anticorps Monoclonal anti-LGALS3BP

LGALS3BP Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2D8E11

N° de cat : 60066-1-Ig

Synonymes

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

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Galerie de données de validation

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  WB analysis of human blood using 60066-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 60066-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 60066-1-Ig

  IP Result of anti-LGALS3BP (IP:60066-1-Ig, 5ug; Detection:60066-1-Ig 1:1000) with human plasma lysate 4000ug.

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  IP experiment of human plasma using 60066-1-Ig

  IP Result of anti-LGALS3BP (IP:60066-1-Ig, 5ug; Detection:60066-1-Ig 1:300) with human plasma lysate 4000ug.

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  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50.

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  IF Staining of A431 using 60066-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LGALS3BP antibody (60066-1-Ig, Clone: 2D8E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu sanguin humain
• Résultats positifs en IP: tissu plasmatique humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la peau humain, tissu de cancer de l'œsophage humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

60066-1-Ig cible LGALS3BP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LGALS3BP Protéine recombinante Ag0294
• Nom complet: lectin, galactoside-binding, soluble, 3 binding protein
• Masse moléculaire calculée: 585 aa, 65 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC002403
• Symbole du gène: LGALS3BP
• Identification du gène (NCBI): 3959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ). LGALS3BP has a predicted mass of ∼65 kDa. It is heavily glycosylated and shows an apparent molecular weight of 90 kDa on SDS-PAGE.

Publications

Species	Application	Title
mouse	WB	PLoS Pathog; Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.; Authors - Gang Xu
human	WB	Invest Ophthalmol Vis Sci; LGALS3BP in Microglia Promotes Retinal Angiogenesis Through PI3K/AKT Pathway During Hypoxia.; Authors - Chenyang Zhao
human	WB	Proteomics; Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles; Authors - Yaojie Wang
human	WB	bioRxiv; Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2; Authors - Aswini Panigrahi

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