Anticorps Polyclonal de lapin anti-LGALS9/Galectin-9

• Phare
• Validé par KD/KO

LGALS9/Galectin-9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 17938-1-AP

Synonymes

Galectin 9, LGALS9, Ecalectin, Gal 9, Gal-9

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Galerie de données de validation

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  WB analysis using 17938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17938-1-AP (LGALS9/Galectin-9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 17938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17938-1-AP (Galectin-9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-937 using 17938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells using LGALS9/Galectin-9 antibody (17938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules L02, cellules NIH/3T3, cellules THP-1, cellules U-937
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-937,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

17938-1-AP cible LGALS9/Galectin-9 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LGALS9/Galectin-9 Protéine recombinante Ag12209
• Nom complet: lectin, galactoside-binding, soluble, 9
• Masse moléculaire calculée: 323 aa, 36 kDa
• Poids moléculaire observé: 34-40 kDa
• Numéro d’acquisition GenBank: BC110340
• Symbole du gène: Galectin-9
• Identification du gène (NCBI): 3965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma; Authors - Junju Chen
	WB	Front Mol Neurosci; Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease; Authors - Qinyu Peng
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	WB,IHC	Front Immunol; Galectin-9/TIM-3 as a Key Regulator of Immune Response in Gliomas With Chromosome 1p/19q Codeletion.; Authors - Guanzhang Li
human	WB,IHC	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
human	WB	Stem Cell Res Ther; Mesenchymal stem cells alleviate experimental autoimmune cholangitis through immunosuppression and cytoprotective function mediated by galectin-9.; Authors - Junyu Fan

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