Anticorps Polyclonal de lapin anti-Galectin-9

• Phare
• Validé par KD/KO

Galectin-9 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 17938-1-AP

Synonymes

Ecalectin, Gal 9, Galectin 9, HUAT, LGALS9, LGALS9A, Tumor antigen HOM HD 21

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 17938-1-AP

  WB result of Galectin 9 antibody (17938-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 9 transfected NIH/3T3 cells.

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  WB analysis of U-937 using 17938-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17938-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17938-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 17938-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 17938-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 17938-1-AP

  IP result of anti-Galectin-9(IP:17938-1-AP, 4ug; Detection:17938-1-AP 1:1500) with U-937 cells lysate 2240 ug.

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  IF Staining of HeLa using 17938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 17938-1-AP (Galectin 9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U-937, cellules HeLa, cellules HepG2, cellules HL-60, cellules NIH/3T3, cellules SGC-7901
• Résultats positifs en IP: cellules U-937,
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

17938-1-AP cible Galectin-9 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Galectin-9 Protéine recombinante Ag12209
• Nom complet: lectin, galactoside-binding, soluble, 9
• Masse moléculaire calculée: 323 aa, 36 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC110340
• Symbole du gène: LGALS9
• Identification du gène (NCBI): 3965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

Publications

Species	Application	Title
	WB	Front Mol Neurosci; Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease; Authors - Qinyu Peng
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	WB,IHC	Front Immunol; Galectin-9/TIM-3 as a Key Regulator of Immune Response in Gliomas With Chromosome 1p/19q Codeletion.; Authors - Guanzhang Li
human	WB,IHC	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
human	WB	Stem Cell Res Ther; Mesenchymal stem cells alleviate experimental autoimmune cholangitis through immunosuppression and cytoprotective function mediated by galectin-9.; Authors - Junyu Fan
human	WB,IHC,IP	Immunotherapy; Galectin-9 and PD-L1 antibody blockade combination therapy inhibits tumour progression in pancreatic cancer; Authors - Enliang Li