Anticorps Polyclonal de lapin anti-LGI3

LGI3 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC,ELISA

Conjugaison

Non conjugué

N° de cat : 21919-1-AP

Synonymes

LGI1 like protein 4, LGI3, LGIL4

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Galerie de données de validation

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  WB analysis of HepG2 using 21919-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 21919-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21919-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21919-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21919-1-AP

  Immunofluorescent analysis of HepG2 cells using 21919-1-AP (LGI3 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-Rabbit IgG (red).

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  FC experiment of HepG2 using 21919-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug LGI3 antibody (21919-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules SMMC-7721, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21919-1-AP cible LGI3 dans les applications de WB, IHC, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LGI3 Protéine recombinante Ag16257
• Nom complet: leucine-rich repeat LGI family, member 3
• Masse moléculaire calculée: 548 aa, 62 kDa
• Poids moléculaire observé: 55-62 kDa
• Numéro d’acquisition GenBank: BC142997
• Symbole du gène: LGI3
• Identification du gène (NCBI): 203190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.