Anticorps Polyclonal de lapin anti-LIAS

LIAS Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(74)

Avis (1)

N° de cat : 11577-1-AP

Synonymes

HUSSY 01, LAS, LIAS, Lip syn, LIP1, Lipoate synthase, Lipoic acid synthase, lipoic acid synthetase, Lipoyl synthase, mitochondrial, LS

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Galerie de données de validation

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  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11577-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11577-1-AP (LIAS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11577-1-AP (LIAS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11577-1-AP (LIAS antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HepG2, cellules K-562, cellules Raji, tissu cérébral de souris, tissu cérébral humain, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 70 publications below
• IHC: See 11 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

11577-1-AP cible LIAS dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIAS Protéine recombinante Ag2162
• Nom complet: lipoic acid synthetase
• Masse moléculaire calculée: 372 aa, 42 kDa
• Poids moléculaire observé: 34-42 kDa
• Numéro d’acquisition GenBank: BC023635
• Symbole du gène: LIAS
• Identification du gène (NCBI): 11019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).

Publications

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
human,mouse	WB,IHC	Adv Sci (Weinh); Enzalutamide Sensitizes Castration-Resistant Prostate Cancer to Copper-Mediated Cell Death; Authors - Xiang Gao
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
mouse	WB	Small; A Copper-Manganese Based Nanocomposite Induces Cuproptosis and Potentiates Anti-Tumor Immune Responses; Authors - Luoyingzi Xie
mouse	WB	Biomaterials; Magnesium hexacyanoferrate mitigates sepsis-associated encephalopathy through inhibiting microglial activation and neuronal cuproptosis; Authors - Yabing Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (10-01-2025)

Excellent antibody with perfect detection by chemiluminescence. Have used at 1:1000 and 1:2000 dilutions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human In vitro Cancer Cells

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