Anticorps Monoclonal anti-LIAS

LIAS Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

3B5G7

N° de cat : 67298-1-PBS

Synonymes

3B5G7, EC:2.8.1.8, HUSSY 01, HUSSY-01, LAS

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Galerie de données de validation

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  WB analysis of LNCaP using 67298-1-Ig (same clone as 67298-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

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  WB analysis using 67298-1-Ig (same clone as 67298-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67298-1-Ig (same clone as 67298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67298-1-Ig (LIAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67298-1-PBS cible LIAS dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LIAS Protéine recombinante Ag27286
• Nom complet: lipoic acid synthetase
• Masse moléculaire calculée: 372 aa, 42 kDa
• Poids moléculaire observé: 34-42 kDa
• Numéro d’acquisition GenBank: BC023635
• Symbole du gène: LIAS
• Identification du gène (NCBI): 11019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).

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