Anticorps Polyclonal de lapin anti-LIF

• Phare
• Validé par KD/KO

LIF Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 26757-1-PBS

Synonymes

Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor

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Galerie de données de validation

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  WB analysis using 26757-1-AP (same clone as 26757-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 26757-1-AP (same clone as 26757-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 26757-1-AP (same clone as 26757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 26757-1-AP (same clone as 26757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using LIF antibody (26757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 26757-1-AP (same clone as 26757-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LIF antibody (26757-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26757-1-PBS cible LIF dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIF Protéine recombinante Ag25076
• Nom complet: leukemia inhibitory factor (cholinergic differentiation factor)
• Masse moléculaire calculée: 202 aa, 22 kDa
• Poids moléculaire observé: 22 kDa, 32 kDa
• Numéro d’acquisition GenBank: BC093733
• Symbole du gène: LIF
• Identification du gène (NCBI): 3976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF is a glycoprotein with variable molecular weight (38-67 kDa) due to differential glycosylation. (PMID: 8262236, PMID: 35204717)

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