Anticorps Polyclonal de lapin anti-DNA Ligase I

• Phare
• Validé par KD/KO

DNA Ligase I Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 18051-1-AP

Synonymes

DNA ligase 1, DNA Ligase I, LIG1, ligase I, DNA, ATP dependent

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Galerie de données de validation

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  WB analysis using 18051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18051-1-AP (DNA Ligase I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18051-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18051-1-AP (DNA Ligase I antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18051-1-AP

  IP result of anti-DNA Ligase I (IP:18051-1-AP, 4ug; Detection:18051-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human spleen using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules Jurkat
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu splénique humain, tissu cérébral humain, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

18051-1-AP cible DNA Ligase I dans les applications de WB, IHC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNA Ligase I Protéine recombinante Ag12508
• Nom complet: ligase I, DNA, ATP-dependent
• Masse moléculaire calculée: 919 aa, 102 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC108318
• Symbole du gène: LIG1
• Identification du gène (NCBI): 3978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 18051-1-AP is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.

Publications

Species	Application	Title
mouse	WB	Nature; eccDNAs are apoptotic products with high innate immunostimulatory activity; Authors - Yuangao Wang
human	WB,IHC	Mol Cancer; hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma; Authors - Lei Xu
mouse	WB	Nat Commun; Cas9-induced large deletions and small indels are controlled in a convergent fashion.; Authors - Michael Kosicki
human	WB,IP	J Clin Invest; Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.; Authors - Patrick Maffucci ; KO Validated
rat	WB	Proc Natl Acad Sci U S A; Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.; Authors - Shahnaz Masani ; KO Validated
mouse	WB	Proc Natl Acad Sci U S A; Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.; Authors - Guangqing Lu ; KO Validated

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