Anticorps Monoclonal anti-DNA ligase III/LIG3

DNA ligase III/LIG3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B10B3

N° de cat : 68810-1-PBS

Synonymes

LIG3, 1B10B3, DNA ligase 3, DNA ligase III, EC:6.5.1.1

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Galerie de données de validation

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  WB analysis using 68810-1-Ig (same clone as 68810-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68810-1-Ig (DNA ligase III/LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68810-1-Ig (same clone as 68810-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50683-2

  Cytometric bead array standard curve of MP50683-2, DNA ligase III/LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-3-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

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  Cytometric bead array standard curve of MP50683-3

  Cytometric bead array standard curve of MP50683-3, DNA ligase III/LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-2-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

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Informations sur le produit

68810-1-PBS cible DNA ligase III/LIG3 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DNA ligase III/LIG3 Protéine recombinante Ag31484
• Nom complet: ligase III, DNA, ATP-dependent
• Masse moléculaire calculée: 100-110 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC068005
• Symbole du gène: LIG3
• Identification du gène (NCBI): 3980
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

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