Anticorps Monoclonal anti-LIG4

LIG4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H6C11

N° de cat : 66705-1-PBS

Synonymes

DNA ligase IV, DNA ligase 4, EC:6.5.1.1, LIG 4, Ligase IV

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Galerie de données de validation

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  WB analysis of PC-3 using 66705-1-Ig (same clone as 66705-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66705-1-Ig (same clone as 66705-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66705-1-Ig (same clone as 66705-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66705-1-Ig (same clone as 66705-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66705-1-Ig (same clone as 66705-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66705-1-Ig (same clone as 66705-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66705-1-Ig (same clone as 66705-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66705-1-Ig (LIG4 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66705-1-PBS cible LIG4 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LIG4 Protéine recombinante Ag3385
• Nom complet: ligase IV, DNA, ATP-dependent
• Masse moléculaire calculée: 911 aa, 104 kDa
• Poids moléculaire observé: 100-104 kDa
• Numéro d’acquisition GenBank: BC037491
• Symbole du gène: LIG4
• Identification du gène (NCBI): 3981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

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