Anticorps Polyclonal de lapin anti-LIMD1

LIMD1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 28106-1-AP

Synonymes

LIM domains containing 1, LIMD1



Applications testées

Résultats positifs en WBcellules A549, cellules HeLa
Résultats positifs en IPcellules HeLa,
Résultats positifs en IF/ICCcellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

IFSee 1 publications below

Informations sur le produit

28106-1-AP cible LIMD1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène LIMD1 Protéine recombinante Ag27974
Nom complet LIM domains containing 1
Masse moléculaire calculée 72 aa
Poids moléculaire observé 72 kDa
Numéro d’acquisition GenBankNM_014240
Symbole du gène LIMD1
Identification du gène (NCBI) 8994
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIMD1 is a member of the Ajuba family of LIM domain-containing proteins, these kinds of proteins have been shown to play a role in intracellular signaling, transcriptional regulation and cellular differentiation, proliferation and migration (PMID:17174104). LIMD1 predominantly localizes to the cytoplasm especially in the E-cadherin cell-cell adhesive junction, yet also translocates to the nucleus when functions as an RB corepressor (PMID:19060205). And LIMD1 acts as a scaffolding protein to form a PHD-LIMD1-VHL axis, facilitating HIF1α ubiquitination and degradation by the proteasome (PMID:22800800). LIMD1 specifically interacts with retinoblastoma protein (pRB), inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. As a tumor suppressor, LIMD1 blocks tumor growth in vitro and in vivo, and is frequently down-regulated in human lung tumors (PMID:15542589), and it also be detected in normal and breast cancer tissues. LIMD1 protein exists several phosphorylation sites, which may affect its theoretical molecular weight when tested. Proteintech's 28106-1-AP antibody was generated by 133 amino acids, it could recognize the full-length protein in applications.

Protocole

Product Specific Protocols
WB protocol for LIMD1 antibody 28106-1-APDownload protocol
IF protocol for LIMD1 antibody 28106-1-APDownload protocol
IP protocol for LIMD1 antibody 28106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Cell Biol

Paxillin phase separation promotes focal adhesion assembly and integrin signaling

Authors - Peigang Liang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Paul (Verified Customer) (11-24-2022)

Clear specific signal just above 70kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Different lung cell lines (A549, H1299)
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