Anticorps Monoclonal anti-LIMK1

• Phare
• Validé par KD/KO

LIMK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B11E9

N° de cat : 67974-1-PBS

Synonymes

LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 67974-1-Ig (same clone as 67974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  WB analysis using 67974-1-Ig (same clone as 67974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67974-1-Ig (same clone as 67974-1-PBS)

  WB result of LIMK1 antibody (67974-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMK1 transfected HeLa cells. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 67974-1-Ig (same clone as 67974-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67974-1-Ig (same clone as 67974-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 67974-1-Ig (same clone as 67974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67974-1-Ig (LIMK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 67974-1-Ig (same clone as 67974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67974-1-Ig (LIMK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67974-1-Ig (same clone as 67974-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LIMK1 antibody (67974-1-Ig, Clone: 2B11E9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67974-1-PBS cible LIMK1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LIMK1 Protéine recombinante Ag28862
• Nom complet: LIM domain kinase 1
• Masse moléculaire calculée: 647 aa, 73 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC152982
• Symbole du gène: LIMK1
• Identification du gène (NCBI): 3984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.

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