Anticorps Recombinant de lapin anti-LIMK2

LIMK2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230283D10

N° de cat : 82977-1-PBS

Synonymes

LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283D10

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Galerie de données de validation

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  IF Staining of HepG2 using 82977-1-RR (same clone as 82977-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LIMK2 antibody (82977-1-RR, Clone: 230283D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82977-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82977-1-RR (same clone as 82977-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LIMK2 Recombinant antibody (82977-1-RR, Clone:230283D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82977-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82977-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82977-1-RR against Human LIMK2 were performed. The affinity constant is 4.03 nM.

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Informations sur le produit

82977-1-PBS cible LIMK2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LIMK2 Protéine recombinante Ag3011
• Nom complet: LIM domain kinase 2
• Masse moléculaire calculée: 686 aa, 78 kDa
• Numéro d’acquisition GenBank: BC013051
• Symbole du gène: LIMK2
• Identification du gène (NCBI): 3985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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