Anticorps Monoclonal anti-LIN28A

LIN28A Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4F5G6

N° de cat : 60344-1-PBS

Synonymes

Lin-28A, LIN28, Lin 28A, LIN 28, CSDD1

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Galerie de données de validation

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  WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using LIN28 antibody (60344-1-Ig, Clone: 4F5G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60344-1-PBS cible LIN28A dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LIN28A Protéine recombinante Ag2312
• Nom complet: lin-28 homolog (C. elegans)
• Masse moléculaire calculée: 209 aa, 23 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC028566
• Symbole du gène: LIN28
• Identification du gène (NCBI): 79727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. LIN28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic mRNA-binding protein that binds to and enhances the translation of the IGF2 mRNA. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

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