Anticorps Polyclonal de lapin anti-LIN7C

LIN7C Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 14656-1-AP

Synonymes

LIN 7 C, lin 7 homolog C (C. elegans), LIN 7C, LIN7C, MALS 3, MALS3, Mammalian lin seven protein 3, Protein lin 7 homolog C, Veli 3, VELI3, Vertebrate lin 7 homolog 3

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Galerie de données de validation

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  WB analysis of human cerebellum using 14656-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14656-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14656-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14656-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14656-1-AP (LIN7C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de cervelet humain, tissu cérébral humain, tissu rénal humain
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14656-1-AP cible LIN7C dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIN7C Protéine recombinante Ag6265
• Nom complet: lin-7 homolog C (C. elegans)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC053907
• Symbole du gène: LIN7C
• Identification du gène (NCBI): 55327
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Neuroscience; Proteome analysis of actin filament-associated proteins in the postnatal rat cerebellum.; Authors - Shi N N

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Thomas (Verified Customer) (11-17-2020)

Good specifity, also binds canine Lin7c

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116 (human, colon cancer cell line), Caco2 (human, colorectal adenocarcinoma cell line) , MDCK (canine, kidney tubule)

LIN7C Antibody Western Blot validation (1:1000 dilution) in HCT116 (human, colon cancer cell line), Caco2 (human, colorectal adenocarcinoma cell line) , MDCK (canine, kidney tubule) (Cat no:14656-1-AP)