Anticorps Polyclonal de lapin anti-LIN9

LIN9 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 17882-1-AP

Synonymes

TGS, BARA, Beta subunit-associated regulator of apoptosis, hLin-9, HuLin-9

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Galerie de données de validation

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  WB analysis using 17882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17882-1-AP (LIN9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 17882-1-AP

  IP result of anti-LIN9 (IP:17882-1-AP, 4ug; Detection:17882-1-AP 1:1000) with HEK-293 cells lysate 1100ug.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules NIH/3T3, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

17882-1-AP cible LIN9 dans les applications de WB, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIN9 Protéine recombinante Ag11889
• Nom complet: lin-9 homolog (C. elegans)
• Masse moléculaire calculée: 558 aa, 64 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC045625
• Symbole du gène: LIN9
• Identification du gène (NCBI): 286826
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIN9, also named as BARA or TGS, is a 542 amino acid protein, which belongs to the lin9 family. LIN9 localizes in the nucleus and is expressed in thymus and testis. LIN9 acts as a tumor suppressor and inhibits DNA synthesis. LIN9 plays a role in the expression of genes, which required for the G1/S transition.

Publications

Species	Application	Title
		Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen
human	WB,IF,CoIP	Int J Oncol; Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9; Authors - Zhenwei Ma
human	WB	Sci China Life Sci; PMID: 31420851; Authors - Hongna Lai
human	WB	EMBO Mol Med; Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple-negative breast cancer; Authors - Laura Pascual-Reguant
mouse	WB	FEBS Open Bio; The dual role of the DREAM/G2M pathway in non-tumorigenic immortalization of senescent cells; Authors - Jie Tian
human	WB	J Transl Med; Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation; Authors - Qinghui Kan ; KD Validated

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