Anticorps Polyclonal de lapin anti-LIPF

LIPF Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 25889-1-AP

Synonymes

lipase, gastric, HLAL, HGL, GL, Gastric lipase

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Galerie de données de validation

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  WB analysis of MKN-45 using 25889-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 25889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MKN-45, tissu d'estomac de souris
• Résultats positifs en IHC: tissu d'estomac humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25889-1-AP cible LIPF dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIPF Protéine recombinante Ag23149
• Nom complet: lipase, gastric
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC112272
• Symbole du gène: LIPF
• Identification du gène (NCBI): 8513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

gastric lipase, is an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions.

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