Anticorps Polyclonal de lapin anti-LITAF

• Phare
• Validé par KD/KO

LITAF Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16797-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

  IP result of anti-LITAF (IP:16797-1-AP, 4ug; Detection:16797-1-AP 1:600) with HeLa cells lysate 1520ug. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 16797-1-AP (same clone as 16797-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 16797-1-AP (same clone as 16797-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16797-1-AP (LITAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16797-1-PBS cible LITAF dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LITAF Protéine recombinante Ag10269
• Nom complet: lipopolysaccharide-induced TNF factor
• Masse moléculaire calculée: 161 aa, 17 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC008309
• Symbole du gène: LITAF
• Identification du gène (NCBI): 9516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Lipopolysaccharide-induced TNF factor(LITAF) is a transcription factor that identified as a regulator of TNF-alpha gene expression. It may has a role in protein degradation pathways and in tumor necrosis factor alpha(TNF-alpha) gene expression. Also it may regulate the expression of the CCL2/MCP-1 chemokine through NFKB1.Defects in LITAF may be involved in extramammary Paget disease (EMPD) carcinogenesis

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