Anticorps Monoclonal anti-LMOD1

LMOD1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

1D6H2

N° de cat : 60414-1-PBS

Synonymes

1D6H2, Leiomodin1, Leiomodin-1, SM-Lmod, Thyroid-associated ophthalmopathy autoantigen

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Galerie de données de validation

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  WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

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  WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

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  Sample test of MP51375-1

  Sample test of MP51375-1, LMOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60414-2-PBS. Detection antibody: 60414-1-PBS.

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  IHC staining of human colon using 60414-1-Ig (same clone as 60414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60414-1-Ig (LMOD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 60414-1-Ig (same clone as 60414-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using LMOD1 antibody (60414-1-Ig, Clone: 1D6H2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51375-1

  Cytometric bead array standard curve of MP51375-1, LMOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60414-2-PBS. Detection antibody: 60414-1-PBS. Standard:Ag7568. Range: 0.195-200 ng/mL.

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Informations sur le produit

60414-1-PBS cible LMOD1 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LMOD1 Protéine recombinante Ag7568
• Nom complet: leiomodin 1 (smooth muscle)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC001755
• Symbole du gène: LMOD1
• Identification du gène (NCBI): 25802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.

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