Anticorps Polyclonal de lapin anti-LNPEP

LNPEP Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55154-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of human brain using 55154-1-AP (same clone as 55154-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55154-1-AP (same clone as 55154-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 55154-1-AP (same clone as 55154-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 55154-1-AP (same clone as 55154-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

55154-1-PBS cible LNPEP dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: leucyl/cystinyl aminopeptidase
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 117-125 kDa
• Numéro d’acquisition GenBank: NM_005575
• Symbole du gène: LNPEP
• Identification du gène (NCBI): 4012
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LNPEP, also named as OTASE, IRAP, P-LAP and Otase, may be involved in the inactivation of neuronal peptides in the brain. LNPEP cleaves Met-enkephalin and dynorphin. It binds angiotensin IV and may be the angiotensin IV receptor in the brain. It can be detected a protein with molecular mass of 165 kDa in several human tissues including heart, small intestine, kidney and 140 kDa in brain. LNPEP contains 18 potential N-glycosylation sites so that the differential usage of the glycosylation site in the brain causes the difference in the molecular mass of the enzyme.(PMID:11389728). Two forms of LNPEP are purified from human placenta: one is a monomeric, 61-kDa protein and the other is a heterodimeric protein with 64- and 97-kDa subunits(PMID:10601849). It can form a homodimer of 280 kDa(PMID:10601849). This antibody is specific to LNPEP.

{{ptg:RelatedPrimaryAntibodies}}