Anticorps Recombinant de lapin anti-LONP1

LONP1 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240460E5

N° de cat : 83552-3-PBS

Synonymes

240460E5, EC:3.4.21.53, LON, LONP, LONP 1

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Galerie de données de validation

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  IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83552-3-RR (same clone as 83552-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-3-RR, Clone:240460E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00531-3

  Cytometric bead array standard curve of MP00531-3, LONP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83552-3-PBS. Detection antibody: 83552-1-PBS. Standard: Ag7196. Range: 0.938-60 ng/mL.

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  Affinity and Kinetic Characterization of 83552-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83552-3-RR against Human LONP1 were performed. The affinity constant is 1.38 nM.

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Informations sur le produit

83552-3-PBS cible LONP1 dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LONP1 Protéine recombinante Ag7196
• Nom complet: lon peptidase 1, mitochondrial
• Masse moléculaire calculée: 106 kDa
• Numéro d’acquisition GenBank: BC000235
• Symbole du gène: LONP1
• Identification du gène (NCBI): 9361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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