Anticorps Polyclonal de lapin anti-LOX

• Phare
• Validé par KD/KO

LOX Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

32

N° de cat : 17958-1-AP

Synonymes

LOX, lysyl oxidase, Protein lysine 6 oxidase

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Galerie de données de validation

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  WB analysis of Jurkat using 17958-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 17958-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 17958-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17958-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MKN-45 using 17958-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17958-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 17958-1-AP

  IP Result of anti-LOX (IP:17958-1-AP, 3ug; Detection:17958-1-AP 1:500) with mouse skeletal muscle tissue lysate 2000ug.

• 

  IHC staining of human stomach cancer using 17958-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17958-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 17958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 17958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A375, cellules MKN-45, cellules NIH/3T3, cellules SGC-7901, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu oculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 12 publications below
• IF: See 3 publications below

Informations sur le produit

17958-1-AP cible LOX dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LOX Protéine recombinante Ag12479
• Nom complet: lysyl oxidase
• Masse moléculaire calculée: 417 aa, 47 kDa
• Poids moléculaire observé: 32 kDa, 45-47 kDa
• Numéro d’acquisition GenBank: BC074872
• Symbole du gène: LOX
• Identification du gène (NCBI): 4015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.

Publications

Species	Application	Title
		Cell Syst; Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.; Authors - Stephanie A Zlatic
human	WB	Oncogene; Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids; Authors - Hui Zhao
human	IHC,IF	Oncogene; Cell-to-cell heterogeneity of EWSR1-FLI1 activity determines proliferation/migration choices in Ewing sarcoma cells.; Authors - G-A Franzetti
human	IHC	Int J Nanomedicine; Gallbladder Cancer Progression Is Reversed by Nanomaterial-Induced Photothermal Therapy in Combination with Chemotherapy and Autophagy Inhibition.; Authors - Qiang Cai
human	IHC	ACS Biomater Sci Eng; Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.; Authors - Binghui Liu
human	IHC	Biomed Pharmacother; Drug resistance evaluation in novel 3D in vitro model.; Authors - Marta Nowacka