Anticorps Polyclonal de lapin anti-LOX

• Phare
• Validé par KD/KO

LOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17958-1-PBS

Synonymes

Protein-lysine 6-oxidase, short form, Protein-lysine 6-oxidase, long form, Protein-lysine 6-oxidase, lysyl oxidase, EC:1.4.3.13

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Galerie de données de validation

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  WB analysis of Jurkat using 17958-1-AP (same clone as 17958-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 17958-1-AP (same clone as 17958-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of MKN-45 using 17958-1-AP (same clone as 17958-1-PBS)

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 17958-1-AP (same clone as 17958-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 17958-1-AP (same clone as 17958-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 17958-1-AP (same clone as 17958-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 17958-1-AP (same clone as 17958-1-PBS)

  IP result of anti-LOX (IP:17958-1-AP, 3ug; Detection:17958-1-AP 1:500) with mouse skeletal muscle tissue lysate 2000ug. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 17958-1-AP (same clone as 17958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 17958-1-AP (same clone as 17958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 17958-1-AP (same clone as 17958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 17958-1-AP (same clone as 17958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17958-1-PBS cible LOX dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LOX Protéine recombinante Ag12479
• Nom complet: lysyl oxidase
• Masse moléculaire calculée: 417 aa, 47 kDa
• Poids moléculaire observé: 32 kDa, 45-47 kDa
• Numéro d’acquisition GenBank: BC074872
• Symbole du gène: LOX
• Identification du gène (NCBI): 4015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.

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