Anticorps Recombinant de lapin anti-LPP

LPP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250773C9

N° de cat : 86117-3-RR

Synonymes

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

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Galerie de données de validation

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  WB analysis using 86117-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86117-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86117-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPP antibody (86117-3-RR, Clone: 250773C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86117-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86117-3-RR against Human LPP were performed. The affinity constant is 1.18 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, tissu de côlon de rat, tissu de côlon de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86117-3-RR cible LPP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LPP Protéine recombinante Ag19205
• Nom complet: LIM domain containing preferred translocation partner in lipoma
• Masse moléculaire calculée: 612 aa, 66 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC130584
• Symbole du gène: LPP
• Identification du gène (NCBI): 4026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).

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