Anticorps Polyclonal de lapin anti-LPXN

• Phare
• Validé par KD/KO

LPXN Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 11307-1-AP

Synonymes

LDPL, LDLP

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Galerie de données de validation

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  WB analysis using 11307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11307-1-AP (LPXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11307-1-AP

  WB result of LPXN antibody (11307-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPXN transfected PC-3 cells.

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  IP experiment of PC-3 using 11307-1-AP

  IP result of anti-LPXN (IP:11307-1-AP, 4ug; Detection:11307-1-AP 1:5000) with PC-3 cells lysate 1575 ug.

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  IF Staining of PC-3 using 11307-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using LPXN antibody (11307-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules PC-3
• Résultats positifs en IP: cellules PC-3,
• Résultats positifs en IF/ICC: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11307-1-AP cible LPXN dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LPXN Protéine recombinante Ag1845
• Nom complet: leupaxin
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC019035
• Symbole du gène: LPXN
• Identification du gène (NCBI): 9404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).

Publications

Species	Application	Title
mouse	IF	J Mol Cell Cardiol; Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG.; Authors - Xinming Wang
mouse	IHC	Mol Cell Biochem; STAT3 mediates ECM stiffness-dependent progression in ovarian cancer; Authors - Sun Chenchen

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