Anticorps Recombinant de lapin anti-LPXN

• Phare
• Validé par KD/KO

LPXN Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240483G10

N° de cat : 83657-5-PBS

Synonymes

240483G10, LDLP, LDPL

---

Galerie de données de validation

• 

  WB analysis using 83657-5-RR (same clone as 83657-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83657-5-RR (LPXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 83657-5-RR (same clone as 83657-5-PBS)

  WB result of LPXN antibody (83657-5-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPXN transfected PC-3 cells. This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 83657-5-RR (same clone as 83657-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using LPXN antibody (83657-5-RR, Clone: 240483G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83657-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83657-5-RR against Human LPXN were performed. The affinity constant is 0.187 nM.

---

Informations sur le produit

83657-5-PBS cible LPXN dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LPXN Protéine recombinante Ag34956
• Nom complet: leupaxin
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC019035
• Symbole du gène: LPXN
• Identification du gène (NCBI): 9404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).

{{ptg:RelatedPrimaryAntibodies}}