Anticorps Recombinant de lapin anti-LRAT

LRAT Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243058B5

N° de cat : 85628-1-PBS

Synonymes

EC:2.3.1.135, Lecithin retinol acyltransferase, Phosphatidylcholine--retinol O-acyltransferase

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Galerie de données de validation

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  WB analysis using 85628-1-RR (same clone as 85628-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85628-1-RR (LRAT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85628-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85628-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85628-1-RR against Human LRAT were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85628-1-PBS cible LRAT dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LRAT Protéine recombinante Ag3556
• Nom complet: lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
• Masse moléculaire calculée: 230 aa, 26 kDa
• Poids moléculaire observé: 26 kDa, 50-54 kDa
• Numéro d’acquisition GenBank: BC031053
• Symbole du gène: LRAT
• Identification du gène (NCBI): 9227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.

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