Anticorps Polyclonal de lapin anti-LRG1

• Phare
• Validé par KD/KO

LRG1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 13224-1-AP

Synonymes

HMFT1766, LRG, LRG1

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Galerie de données de validation

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  WB analysis of human plasma using 13224-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 13224-1-AP (LRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13224-1-AP (LRG1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13224-1-AP (LRG1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13224-1-AP (LRG1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13224-1-AP (LRG1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13224-1-AP (LRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13224-1-AP (LRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13224-1-AP (LRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13224-1-AP (LRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13224-1-AP (LRG1 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13224-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13224-1-AP (LRG1 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: plasma humain,
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral de souris, tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 11 publications below
• IF: See 8 publications below
• FC: See 1 publications below

Informations sur le produit

13224-1-AP cible LRG1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRG1 Protéine recombinante Ag3964
• Nom complet: leucine-rich alpha-2-glycoprotein 1
• Masse moléculaire calculée: 347 aa, 38 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC034389
• Symbole du gène: LRG1
• Identification du gène (NCBI): 116844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRG1, also known as LRG, is a member of the leucine-rich repeat (LRR) family of proteins, containing eight LRR (leucine-rich) repeats and one LRRCT domain. The gene of LRG1 maps to chromosome 19p13.3, and encodes a 347-amino acid protein with a predicted unmodified molecular weight of 38 kD. The mature form of LRG1 is a secreted glycoprotein which has 312 amino acids and an experimentally determined molecular mass of 45 kD. The LRR family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation. Levels of the LRG protein are markedly elevated in acute appendicitis and therefore could be used as a diagnostic aid.

Publications

Species	Application	Title
human		Small Methods; Highly Sensitive Exosome Detection for Early Diagnosis of Pancreatic Cancer Using Immunoassay Based on Hierarchical Surface-Enhanced Raman Scattering Substrate.; Authors - Juan Li
mouse	IF	Kidney Int; Gene expression profiles of glomerular endothelial cells support their role in the glomerulopathy of diabetic mice.; Authors - Jia Fu
mouse	WB,IF,FC	Diabetes; A Multifunctional Role of Leucine-Rich α-2-Glycoprotein 1 in Cutaneous Wound Healing Under Normal and Diabetic Conditions.; Authors - Chenghao Liu ; KO Validated
human	WB	Oncotarget; Leucine-rich α-2-glycoprotein promotes TGFβ1-mediated growth suppression in the Lewis lung carcinoma cell lines.; Authors - Norihiko Takemoto
human	IHC	Cancers (Basel); LRG1 Promotes Metastatic Dissemination of Melanoma through Regulating EGFR/STAT3 Signalling.; Authors - Yuet Ping Kwan
human	IHC,IF	J Biomed Sci; LRG1: an emerging player in disease pathogenesis.; Authors - Carlotta Camilli

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