Anticorps Polyclonal de lapin anti-LRP1

LRP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 26106-1-AP

Synonymes

APR, Apolipoprotein E receptor, APOER, Alpha-2-macroglobulin receptor, Alpha 2 macroglobulin receptor

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Galerie de données de validation

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  WB analysis using 26106-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26106-1-AP (LRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26106-1-AP

  IP result of anti-LRP1 (IP:26106-1-AP, 4ug; Detection:26106-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse brain using 26106-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26106-1-AP (LRP1 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

26106-1-AP cible LRP1 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRP1 Protéine recombinante Ag23098
• Nom complet: low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)
• Masse moléculaire calculée: 505 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC045107
• Symbole du gène: LRP1
• Identification du gène (NCBI): 4035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRP1 (Prolow-density lipoprotein receptor-related protein 1), also known as A2MR, is a multifunctional cell surface receptor that interacts through its cytoplasmic tail with adaptor and scaffold proteins that participate in cellular signaling. It is synthesized in the endoplasmic reticulum as a transmembrane glycosylated precursor that migrates with an apparent molecular mass of about 600 kd on SDS-polyacrylamide gels. After it reaches the Golgi complex, the protein is cleaved to generate two subunits with apparent molecular masses of approximately 515 and 85 kd respectively (PMID: 2112085). It involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Acts as an LRPAP1 alpha-2-macroglobulin receptor. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission (PMID:12888553).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,CoIP	Cancer Lett; LRP1 induces anti-PD-1 resistance by modulating the DLL4-NOTCH2-CCL2 axis and redirecting M2-like macrophage polarisation in bladder cancer; Authors - Hansen Lin
mouse	CoIP	Diabetes Obes Metab; Multiomics analysis of O-GlcNAcylation in podocytes of diabetic kidney disease; Authors - Yun Zou

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