Anticorps Polyclonal de lapin anti-LRP5

LRP5 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 24899-1-AP

Synonymes

BMND1, EVR1, EVR4, HBM, LR3, LRP 5, LRP5, LRP7, OPPG, OPS, OPTA1, VBCH2

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Galerie de données de validation

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  WB analysis of HeLa using 24899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24899-1-AP (LRP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24899-1-AP (LRP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24899-1-AP (LRP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 24899-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24899-1-AP (LRP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu de cancer du foie humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

24899-1-AP cible LRP5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRP5 Protéine recombinante Ag19034
• Nom complet: low density lipoprotein receptor-related protein 5
• Masse moléculaire calculée: 1615 aa, 179 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC150595
• Symbole du gène: LRP5
• Identification du gène (NCBI): 4041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Low-density lipoprotein receptor-related protein 5 (LRP5) is a single-pass type I membrane protein with EGF-like and LDLR domains in the extracellular domain. LRP5 and LRP6 act as coreceptors with Frizzled for Wnt. The complex of Wnt-Fzd-LRP5-LRP6 triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. LRP5 plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in LRP5 gene.

Publications

Species	Application	Title
human	WB	Stem Cell Res Ther; LINC01119 negatively regulates osteogenic differentiation of mesenchymal stem cells via the Wnt pathway by targeting FZD4.; Authors - Hongwei Gao
mouse	WB,IHC,IF,IP	Front Pharmacol; Sini San Inhibits Chronic Psychological Stress-Induced Breast Cancer Stemness by Suppressing Cortisol-Mediated GRP78 Activation.; Authors - Yifeng Zheng
mouse	WB	Calcif Tissue Int; Coenzyme Q10 Protects Against Hyperlipidemia-Induced Osteoporosis by Improving Mitochondrial Function via Modulating miR-130b-3p/PGC-1α Pathway; Authors - Meng Meng
rat	WB	Pharm Biol; Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.; Authors - Ying Xu
human	IHC, WB	Chin J Integr Med; Babao Dan () Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway.; Authors - Meng-Xuan Gui
human	IHC	J Orthop Sci; Wnt signaling pathway correlates with ossification of the spinal ligament: A microRNA array and immunohistochemical study.; Authors - Takafumi Yayama

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