Anticorps Polyclonal de lapin anti-LRP5

LRP5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24899-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 24899-1-AP (same clone as 24899-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24899-1-AP (LRP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24899-1-AP (LRP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24899-1-AP (LRP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hepatocirrhosis using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 24899-1-AP (same clone as 24899-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24899-1-AP (LRP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24899-1-PBS cible LRP5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRP5 Protéine recombinante Ag19034
• Nom complet: low density lipoprotein receptor-related protein 5
• Masse moléculaire calculée: 1615 aa, 179 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC150595
• Symbole du gène: LRP5
• Identification du gène (NCBI): 4041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Low-density lipoprotein receptor-related protein 5 (LRP5) is a single-pass type I membrane protein with EGF-like and LDLR domains in the extracellular domain. LRP5 and LRP6 act as coreceptors with Frizzled for Wnt. The complex of Wnt-Fzd-LRP5-LRP6 triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. LRP5 plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in LRP5 gene.

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