Anticorps Polyclonal de lapin anti-LRPPRC

• Phare
• Validé par KD/KO

LRPPRC Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

Avis (1)

N° de cat : 21175-1-AP

Synonymes

130 kDa leucine rich protein, CLONE 23970, GP130, LRP 130, LRP130, LRPPRC, LSFC

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Galerie de données de validation

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  WB analysis using 21175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21175-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21175-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21175-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21175-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21175-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 21175-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 21175-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21175-1-AP

  IP Result of anti-LRPPRC (IP:21175-1-AP, 5ug; Detection:21175-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human kidney using 21175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 21175-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 21175-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of U2OS using 21175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of U2OS using 21175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of HepG2 using 21175-1-AP

  Immunofluorescent analysis of HepG2 cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HEK-293 using 21175-1-AP

  Immunofluorescent analysis of HEK-293 cells using 21175-1-AP (LRPPRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 21175-1-AP

  Immunofluorescent analysis of Hela cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HEK-293 using 21175-1-AP

  Immunofluorescent analysis of 293 cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HeLa, cellules HepG2, cellules PC-12, cellules SH-SY5Y, tissu cardiaque de rat, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS, cellules HEK-293, cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Informations sur le produit

21175-1-AP cible LRPPRC dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRPPRC Protéine recombinante Ag15452
• Nom complet: leucine-rich PPR-motif containing
• Masse moléculaire calculée: 1394 aa, 158 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC050311
• Symbole du gène: LRPPRC
• Identification du gène (NCBI): 10128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Publications

Species	Application	Title
mouse	IP	Nat Biotechnol; Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.; Authors - Xue-Hai Liang
mouse	IF,WB,CoIP	Nat Commun; Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA; Authors - Ayesha Sen
human	WB,IF	Nat Commun; A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.; Authors - Peng Sun
human	WB	Genome Biol; Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes; Authors - Iliana Soto ; KO Validated
human	IHC,CoIP	Redox Biol; LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.; Authors - Wen-Su Wei
human	WB	EMBO J; Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.; Authors - Yanni Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angela (Verified Customer) (10-09-2018)

Worked well with 20 ug whole cell lysate run on 7% Tris-Acetate SDS-PAGE. Clean band seen between 120-150 kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:6000
• Cell Tissue Type: HEK293 cells

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