Anticorps Recombinant de lapin anti-LRRC32

LRRC32 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250611B1

N° de cat : 86059-2-RR

Synonymes

Garpin, D11S833E, GARP, Leucine-rich repeat-containing protein 32, Transforming growth factor beta activator LRRC32

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Galerie de données de validation

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  WB analysis of HUVEC using 86059-2-RR

  HUVEC cells were subjected to SDS PAGE followed by western blot with 86059-2-RR (LRRC32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 86059-2-RR

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 86059-2-RR (LRRC32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86059-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86059-2-RR against Human LRRC32 were performed. The affinity constant is 1.40 nM.

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Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain, cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86059-2-RR cible LRRC32 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LRRC32 Protéine recombinante Ag23423
• Nom complet: leucine rich repeat containing 32
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72-80 kDa
• Numéro d’acquisition GenBank: BC070079
• Symbole du gène: LRRC32
• Identification du gène (NCBI): 2615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRRC32, also known as GARP, is a transmembrane protein of 662 amino acids, the extracellular portion of which contains 20 leucine-rich repeats (PMID: 8180135). LRRC32 is a cell surface receptor on regulatory T-lymphocytes (Treg cells), platelets, hepatic stellate cells and certain cancer cells (PMID: 27054568). It has been demonstrated as a Treg-specific activation marker (PMID: 20137067). LRRC32 is critical for the surface expression of latent TGF-β by binding to the complex and functioning as its cell surface receptor (PMID: 19651619).

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