Anticorps Recombinant de lapin anti-LRRC47

LRRC47 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240741A6

N° de cat : 83693-3-PBS

Synonymes

Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6

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Galerie de données de validation

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  WB analysis using 83693-3-RR (same clone as 83693-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83693-3-RR (LRRC47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 83693-3-RR (same clone as 83693-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using LRRC47 antibody (83693-3-RR, Clone: 240741A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83693-3-RR (same clone as 83693-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug LRRC47 Recombinant antibody (83693-3-RR, Clone:240741A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83693-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83693-3-RR against Human LRRC47 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83693-3-PBS cible LRRC47 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LRRC47 Protéine recombinante Ag19736
• Nom complet: leucine rich repeat containing 47
• Masse moléculaire calculée: 583 aa, 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC031301
• Symbole du gène: LRRC47
• Identification du gène (NCBI): 57470
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

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