Anticorps Polyclonal de lapin anti-LRRC8C

LRRC8C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 21601-1-AP

Synonymes

Volume-regulated anion channel subunit LRRC8C, Leucine-rich repeat-containing protein 8C, FAD158, AD158

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Galerie de données de validation

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  WB analysis using 21601-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 21601-1-AP (LRRC8C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 21601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21601-1-AP (LRRC8C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 21601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21601-1-AP (LRRC8C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21601-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LRRC8C antibody (21601-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules THP-1,
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

21601-1-AP cible LRRC8C dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRRC8C Protéine recombinante Ag16213
• Nom complet: leucine rich repeat containing 8 family, member C
• Masse moléculaire calculée: 803 aa, 92 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC113973
• Symbole du gène: LRRC8C
• Identification du gène (NCBI): 84230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRRC8C (Leucine-rich repeat-containing protein 8C), also known as AD158, is a non-essential component of the volume-regulated anion channel (VRAC, also known as VSOAC channel), which is required to maintain a constant cell volume in response to extracellular or intracellular permeability changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. The VRAC channel also mediates transport of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol (PMID:24790029; 26824658; 28193731).

Publications

Species	Application	Title
mouse	WB	Nat Commun; TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer; Authors - Hongwei Lv
rat	IF	Diabetes Metab J; Single-Cell Landscape and a Macrophage Subset Enhancing Brown Adipocyte Function in Diabetes; Authors - Junfei Gu
human	WB	bioRxiv; LRRC8 complexes are adenosine nucleotide release channels regulating platelet activation and arterial thrombosis; Authors - John D Tranter
mouse	WB	bioRxiv; Lysosomal LRRC8 complex regulates lysosomal pH, morphology and systemic glucose metabolism; Authors - Ashutosh Kumar
human	WB	Sci Adv; 2'3'-cGAMP interactome identifies 2'3'-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity; Authors - Yu Deng
human	WB	Sci Adv; Regulation of volume-regulated anion channels alters sensitivity to platinum chemotherapy; Authors - Lily Elizabeth R Feldman

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joshua (Verified Customer) (08-16-2021)

This antibody was incubated at 4 degrees C O/N, in 5% Milk, and at a dilution ratio of 1:1,000. A secondary antibody at a dilution ratio of 1:5,000 was used. The acquired western blot image was achieved at an exposure time of 60 seconds. This product was vital in visualizing LRRC8c in both HUVECs and mouse lung tissue.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HUVECs and Mouse Lung Tissue

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