Anticorps Recombinant de lapin anti-LRRFIP1

LRRFIP1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242161C9

N° de cat : 84778-4-PBS

Synonymes

242161C9, FLAP 1, FLIIAP1, GC binding factor 2, GC-binding factor 2

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Galerie de données de validation

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  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 84778-4-RR (same clone as 84778-4-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRRFIP1 antibody (84778-4-RR, Clone: 242161C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

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Informations sur le produit

84778-4-PBS cible LRRFIP1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LRRFIP1 Protéine recombinante Ag34760
• Nom complet: leucine rich repeat (in FLII) interacting protein 1
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC108914
• Symbole du gène: LRRFIP1
• Identification du gène (NCBI): 9208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

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