Anticorps Polyclonal de lapin anti-LRSAM1

• Phare
• Validé par KD/KO

LRSAM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24666-1-PBS

Synonymes

RING-type E3 ubiquitin transferase LRSAM1, RIFLE, Leucine-rich repeat and sterile alpha motif-containing protein 1, hTAL, EC:2.3.2.27

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Galerie de données de validation

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  WB analysis of Jurkat using 24666-1-AP (same clone as 24666-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 24666-1-AP (same clone as 24666-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 24666-1-AP (same clone as 24666-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 24666-1-AP (same clone as 24666-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRSAM1 antibody (24666-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24666-1-PBS cible LRSAM1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRSAM1 Protéine recombinante Ag20415
• Nom complet: leucine rich repeat and sterile alpha motif containing 1
• Masse moléculaire calculée: 723 aa, 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC009239
• Symbole du gène: LRSAM1
• Identification du gène (NCBI): 90678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LRSAM1 also named as hTAL, RIFLE orTsg101 associated ligase is a 723 amino acid protein, which contains 6 LRR repeats, 1 Ring-type zinc finger and 1 SAM domain. LRSAM1 is localizes in cytoplasm and highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. As a E3 ubiquitin protein ligase, LRSAM1 mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic and exocytic cargos, LRSAM1 gene mutation has an association with Charcot Maire Tooth disease 2P.

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