Anticorps Polyclonal de lapin anti-Perilipin 5

• Phare
• Validé par KD/KO

Perilipin 5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(24)

N° de cat : 26951-1-AP

Synonymes

LSDP5, PLIN5, Perilipin-5, PAT-1, OXPAT

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Galerie de données de validation

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  WB analysis using 26951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26951-1-AP

  WB result of Perilipin 5 antibody (26951-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Perilipin 5 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 26951-1-AP

  WB result of Perilipin 5 antibody (26951-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Perilipin 5 transfected MCF-7 cells.

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  WB analysis of mouse liver using 26951-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 26951-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brown adipose using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu adipeux brun de souris, tissu cardiaque de souris, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

26951-1-AP cible Perilipin 5 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Perilipin 5 Protéine recombinante Ag25272
• Nom complet: lipid storage droplet protein 5
• Masse moléculaire calculée: 463 aa, 51 kDa
• Poids moléculaire observé: 51-55 kDa
• Numéro d’acquisition GenBank: BC131524
• Symbole du gène: Perilipin 5
• Identification du gène (NCBI): 440503
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.

Publications

Species	Application	Title
mouse	WB	J Mol Cell Biol; Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice; Authors - Xinyu Bao
mouse	WB	EMBO Rep; miR-183 and miR-96 orchestrate both glucose and fat utilization in skeletal muscle.; Authors - Hui Wang
mouse	WB	Int J Mol Sci; Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression.; Authors - Miljenko V Panajatovic
mouse	WB	Int Immunopharmacol; Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway.; Authors - Kang Huo
human	WB,IF	Hum Cell; Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.; Authors - Hsueh-Chun Wang
mouse	WB	Biochem Biophys Res Commun; Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.; Authors - Jie Feng

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