Anticorps Monoclonal anti-LSM12

• Phare
• Validé par KD/KO

LSM12 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E3G3

N° de cat : 67029-1-PBS

Synonymes

Protein LSM12, 2E3G3

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Galerie de données de validation

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  WB analysis using 67029-1-Ig (same clone as 67029-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67029-1-Ig (LSM12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67029-1-Ig (same clone as 67029-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug LSM12 Monoclonal antibody (67029-1-Ig, Clone:2E3G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67029-1-PBS cible LSM12 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LSM12 Protéine recombinante Ag28782
• Nom complet: LSM12 homolog (S. cerevisiae)
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC044587
• Symbole du gène: LSM12
• Identification du gène (NCBI): 124801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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