Anticorps Polyclonal de lapin anti-LSM14A

• Phare
• Validé par KD/KO

LSM14A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 18336-1-AP

Synonymes

Protein FAM61A, hRAP55A, hRAP55, FAM61A, C19orf13

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Galerie de données de validation

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  WB analysis using 18336-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18336-1-AP

  WB result of LSM14A antibody (18336-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LSM14A transfected A549 cells.

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  WB analysis of PC-3 using 18336-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18336-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18336-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18336-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18336-1-AP (LSM14A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18336-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18336-1-AP (LSM14A antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 18336-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18336-1-AP (LSM14A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat, cellules PC-3
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 10 publications below
• CoIP: See 1 publications below

Informations sur le produit

18336-1-AP cible LSM14A dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LSM14A Protéine recombinante Ag12997
• Nom complet: LSM14A, SCD6 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 463 aa, 51 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC016842
• Symbole du gène: LSM14A
• Identification du gène (NCBI): 26065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LSm14A was originally discovered to be a component of the P-bodies, it is a member of the LSm family of proteins, which have been shown to be involved in RNA processing events. The N terminus of LSm14A is a conserved LSm domain, whereas the C terminus contains two domains called "DFDF box" and "FDF_TFG box", respectively.

Publications

Species	Application	Title
human	IF	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
human	IF	Acta Pharmacol Sin; NSCLC cells sustain phase separation of cytoplasmic membrane-less organelles to protect themselves against cisplatin treatment; Authors - Ning-Ning Li
human	WB,IF	Elife; Selective sorting of microRNAs into exosomes by phase-separated YBX1 condensates.; Authors - Xiao-Man Liu
mouse	IF	Cell Rep; The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation; Authors - Nicolas Curdy
human	IF	Adv Sci (Weinh); Oleic Acid Dissolves cGAS-DNA Phase Separation to Inhibit Immune Surveillance; Authors - Lina Wang
human	IF	Nucleic Acids Res; RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules; Authors - Vladimir Majerciak

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