Anticorps Polyclonal de lapin anti-LSM2

LSM2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 32074-1-AP

Synonymes

C6orf28, G7B, Protein G7b, snRNP, snRNP core Sm-like protein Sm-x5

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Galerie de données de validation

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  WB analysis using 32074-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32074-1-AP (LSM2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 32074-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32074-1-AP (LSM2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules K-562, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32074-1-AP cible LSM2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LSM2 Protéine recombinante Ag34865
• Nom complet: LSM2 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC009192
• Symbole du gène: LSM2
• Identification du gène (NCBI): 57819
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing (PMID:15075370). Lsm2-Lsm8 (Lsm stands for Like Sm), binds and stabilizes the 3′ end of the spliceosomal U6 snRNA Lsm proteins facilitate the annealing of U6 snRNA with U4 snRNA in vitro and interact with the U4/U6 annealing factor Prp24, suggesting that Lsm proteins function in U4/U6 snRNP formation in vivo (PMID:15485930, 17251193).

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