Anticorps Recombinant de lapin anti-LUC7L

• Phare
• Validé par KD/KO

LUC7L Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240311B10

N° de cat : 83585-1-PBS

Synonymes

Putative RNA-binding protein Luc7-like 1, LUC7L1, LUC7B1, LUC7 LIKE, hLuc7B1

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Galerie de données de validation

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  WB analysis using 83585-1-RR (same clone as 83585-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83585-1-RR (LUC7L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

  WB result of LUC7L antibody (83585-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LUC7L transfected HepG2 cells. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LUC7L antibody (83585-1-RR, Clone: 240311B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83585-1-RR (same clone as 83585-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LUC7L Recombinant antibody (83585-1-RR, Clone:240311B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83585-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83585-1-RR against Human LUC7L were performed. The affinity constant is 99.9 pM.

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Informations sur le produit

83585-1-PBS cible LUC7L dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LUC7L Protéine recombinante Ag9170
• Nom complet: LUC7-like (S. cerevisiae)
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 38 kDa and 44 kDa
• Numéro d’acquisition GenBank: BC065198
• Symbole du gène: LUC7L
• Identification du gène (NCBI): 55692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LUC7L, also known as LUC7L1, belongs to the Luc7 family. LUC7L encodes a putative RNA binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex, which is normally required for 5` splice site selection (PMID: 15474286). LUC7L is widely expressed. LUC7L has 3 isoforms with the molecular mass of 38, 42 and 44 kDa.

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