Anticorps Polyclonal de lapin anti-LXN

LXN Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (2)

N° de cat : 13056-1-AP

Synonymes

ECI, Latexin, Protein MUM, TCI

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Galerie de données de validation

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  WB analysis using 13056-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13056-1-AP (LXN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13056-1-AP

  IP result of anti-LXN (IP:13056-1-AP, 4ug; Detection:13056-1-AP 1:500) with mouse lung tissue lysate 5160ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 13056-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13056-1-AP (LXN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu pulmonaire de rat
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

13056-1-AP cible LXN dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LXN Protéine recombinante Ag3726
• Nom complet: latexin
• Masse moléculaire calculée: 222 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC005346
• Symbole du gène: LXN
• Identification du gène (NCBI): 56925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Latexin, also named as LTXN, LXN, ECI, MUM and TCI, is hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. It may function as a neuroprotective mechanism against tau toxicity.

Publications

Species	Application	Title
mouse	WB,IF	Brain Res; Astrocytic neuroprotection through induction of cytoprotective molecules; a proteomic analysis of mutant P301S tau-transgenic mouse.; Authors - Yata Kenichiro K
mouse	WB	Cell Rep; Latexin regulates sex dimorphism in hematopoiesis via gender-specific differential expression of microRNA 98-3p and thrombospondin 1; Authors - Xiaojing Cui
mouse	IF	Nat Neurosci; Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice; Authors - Timothy M O'Shea
mouse	WB,IF	Int J Biol Macromol; Carboxypeptidase inhibitor Latexin (LXN) regulates intestinal organogenesis and intestinal remodeling involved in intestinal injury repair in mice; Authors - Lingzhu Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

It works for IP study.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

FH

Kyosuke (Verified Customer) (06-05-2019)

It works for WB and IP very well.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: aorta, HUVECs, RASMC, 293T

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