Anticorps Monoclonal anti-LXN

LXN Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E5A5

N° de cat : 66957-1-PBS

Synonymes

TCI, Protein MUM, Latexin, ECI, 3E5A5

---

Galerie de données de validation

• 

  WB analysis of pig brain using 66957-1-Ig (same clone as 66957-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66957-1-Ig (same clone as 66957-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66957-1-Ig (same clone as 66957-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66957-1-Ig (same clone as 66957-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit brain using 66957-1-Ig (same clone as 66957-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP50023-1

  Sandwich ELISA standard curve ofMP50023-1, LXN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66957-1-PBS. Detection antibody: HRP-conjugated 66957-2-PBS. Standard: Ag27922. Range: 0.313-20 ng/mL.

• 

  IHC staining of human heart using 66957-1-Ig (same clone as 66957-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66957-1-Ig (same clone as 66957-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LXN antibody (66957-1-Ig, Clone: 3E5A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LXN (66957-1-Ig, Clone:3E5A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66957-1-PBS cible LXN dans les applications de WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc

• Réactivité: Humain, Lapin, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LXN Protéine recombinante Ag27922
• Nom complet: latexin
• Masse moléculaire calculée: 222 aa, 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC005346
• Symbole du gène: LXN
• Identification du gène (NCBI): 56925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Latexin, also named as LTXN, LXN, ECI, MUM and TCI, is hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. It may function as a neuroprotective mechanism against tau toxicity.

{{ptg:RelatedPrimaryAntibodies}}