Anticorps Polyclonal de lapin anti-LY96/MD2

LY96/MD2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 11784-1-AP

Synonymes

LY96, MD2, ESOP 1, ESOP1, ESOP-1

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Galerie de données de validation

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  WB analysis using 11784-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11784-1-AP (LY96/MD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 11784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11784-1-AP (LY96/MD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11784-1-AP (LY96/MD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

11784-1-AP cible LY96/MD2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LY96/MD2 Protéine recombinante Ag2447
• Nom complet: lymphocyte antigen 96
• Masse moléculaire calculée: 160 aa, 18 kDa
• Poids moléculaire observé: 20-26 kDa
• Numéro d’acquisition GenBank: BC020690
• Symbole du gène: MD2
• Identification du gène (NCBI): 23643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LY96, also named as ESOP1 or MD 2, is a small secreted glycoprotein that can bind to both the hydrophobic portion of LPS and to the extracellular domain of TLR4. The interaction between MD-2 and LPS bridges the two TLR4 molecules and induces the dimerization of LPS-MD-2-TLR4, which forms the structural basis for biological functions of TLR4/MD-2 complex. Due to its essential role in mediating the interaction between LPS and TLR4, MD-2 has been extensively explored as a therapeutic target for treatment of inflammatory disorders.

Publications

Species	Application	Title
human	WB	Front Oncol; MD2 Is a Potential Biomarker Associated with Immune Cell Infiltration in Gliomas.; Authors - Mengya Zhao
mouse	WB	J Pharmacol Exp Ther; The protective effect of Amitriptyline on experimental colitis through inhibiting TLR4/MD2 signaling pathway; Authors - Chengcheng Zeng
mouse	WB	J Cardiovasc Transl Res; Macrophage CD36 and TLR4 Cooperation Promotes Foam Cell Formation and VSMC Migration and Proliferation Under Circadian Oscillations.; Authors - Zhen Sun
rat		Molecules; Sal B Alleviates Myocardial Ischemic Injury by Inhibiting TLR4 and the Priming Phase of NLRP3 Inflammasome.; Authors - Yang Hu
human	WB	Oncol Rep; Curcumin inhibits cell proliferation and migration in NSCLC through a synergistic effect on the TLR4/MyD88 and EGFR pathways.; Authors - Lanfeng Zhang
human	WB	J Reprod Immunol; The NLRP3 activation-related signature predicts the diagnosis and indicates immune characteristics in endometriosis; Authors - Weihua Nong

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